Isolation, preservation and revival of Trichoderma Viride in culture media

Abstract

The present study was conducted in the year 2015-2017; different methods for isolation and preservation methods were tested in CABI lab for 2 years to standardize the long term less expensive preservation technique for Trichoderma viride fungus. Multiple serial dilutions and multiple serial dilutions with 0.05% of Twen-20 were tested and proved to be best for isolation on PDA media. For long term, inexpensive storage; different techniques were tested for preservation of Trichoderma viride which were previously proved to be best for short term and long term storage to other group of fungus. Drying method proved to be best for the preservation of Trichoderma viride for long period of time. It is an easy, convenient, economical, and effective tool for the preservation of Trichoderma viride isolate. Longer monitoring of Trichoderma viride isolate and storage would further validate the reliability strains. Also, the stability of Trichoderma viride should be further assessed by molecular parameters. Drying is not only reliable but is very inexpensive and easy to use in any laboratory with few resources