Influence of BAP with TDZ growth regulators on in vitro regeneration in chrysanthemum (Dendranthema grandiflora T.) cv. Marigold

Abstract

An efficient protocol for in vitro regeneration of Chrysanthemum (Dendranthema grandiflora T.) cv. Marigold was developed. In the present study shoot, tips and nodal segments were used as explants. Nodal explants responded better when compared to shoot tips. Explants were cultured on Murashige and Skoog (MS) medium with different concentrations of 6-benzylaminopurine (BAP) and Thidiazuron (TDZ) alone and with combinations. An intermediate level of BAP (1.0 mgL-1) showed its superiority over other concentrations by giving early shoot initiation (12.10 days) with 3.54 5.33 and 6.25 number of shoots per explant. and maximum shoot length (3.25, 5.85 and 6.99 cm) obtained at 30, 60 and 90 days after culture. MS medium supplemented with BAP 1.5 mgL-1 + TDZ 0.4 mgL-1 gave early shoot initiation (12.40 days), maximum number of shoots per explant (5.25, 8.65 and 11.90) and maximum shoot length (4.45, 5.55 and 7.45 cm) at 30, 60 and 90 days after shoot initiation. Satisfactory rooting response was obtained in half strength MS medium supplemented with 0.2 mgL-1 indole butyric acid (IBA).