Studies on in vitro regeneration of orchids (Dendrobium nobile) using shoot explant

Abstract

Shoot tips of Dendrobium sp. were used as explants and inoculated on MS medium at different concentrations. The maximum callus formation (83%) and the maximum number of callus initiated (12.00) were observed in combination of NAA and BAP at 1mgL-1 into MS medium after 80 days of culture. Subcultured callus were inoculated on MS medium supplemented with different combinations of NAA (0.5, 1.0, 1.5, 2.0 mgL-1) and BAP (0.5, 1.0, 1.5, 2.0 mgL-1) for shoot regeneration. The maximum number of shoot (12.00), the highest shoot length (3.613 cm) and the plantlet regeneration were observed in 0.5 mgL-1 NAA + 1.0 mgL-1 BAP after 60 days of culture. Even, the maximum number of root (4.00), the maximum root length (1.627cm) and the maximum regeneration percentage (91.44%) were observed in 0.5 mgL-1 NAA + 1.0 mgL-1 BAP after 60 days of culture. Finally, regenerated plantlets were transferred into half strength MS medium to obtain plants.