Chemical constituents, antioxidant activities and antimicrobial properties of volatile oil from different part of Duranta repens Linn

Abstract

The volatile oil hydrodistilled from leaf, stem, fruit and root of Duranta repens were investigated for their chemical constituents, antioxidant and antimicrobial activity using GC-MS techniques, DPPH radical-scavenging assay and agar-diffusion method respectively. A total of 30, 12, 20 and 35 constituents were identified in the leaf, stem, fruit and root oils corresponding to 92.5%, 99.5%, 95.1% and 92.2% of the whole volatile oil respectively. Palmitic acid (55.7%, root), styrene (52.5%, fruit), p-vinylanisole (26.0%, fruit) and tetracosane (22.2%, stem) were the prominent components identified in the oils. The fruit and stem oils displayed good DPPH free radical-scavenging activity with IC50 value of 29.39µg/mL and 32.02µg/mL respectively, when compared with that of synthetic antioxidants α-tocopherol (81.58µg/mL) and BHA (45.11µg/mL). The oils showed low antibacterial activity and moderate antifungal activity against the test pathogens with inhibition zone diameter (IZD) range between 2.0±0.0 and 8.5±0.5 mm, lower than the reference compounds gentamycin and ketoconazole (IZD range 9.0±1.4-21.0±1.4 mm). The chemical constituents, antifungal potential and DPPH radical scavenging activity of Duranta repens volatile oils suggest they can act as an adjuncts in lubricant, food, cosmetic and medicine.