Isolation, Enrichment and characterisation of Caprine bone marrow derived mesenchymal stem cells

Abstract

Mesenchymal stem cells are now- a-days treated as the cornerstone of the cellular therapy and tissue engineering arena. These omnipresent cells can be isolated from a wide range of sources and can be differentiated and trans-differentiated into many cell types based on specific cellular command. In this study, we had isolated bone marrow derived mesenchymal stem cells from 1-2 years old apparently healthy goats. The heterologous cell population then subjected to magnetic activated cell sorting against CD73+, CD90+, CD105+, CD271+ and CD34- to exclude mesenchymal stem cells from haematopoietic stem cells. The cells were then cultured and incubated at 37 °C in humidified atmosphere with 5% CO2. After 3 passages the cells were tested for alkaline phosphatase activity. Furthermore, the cells were also evaluated based on their morphometric analysis.