article Open AccessTop 10% cited
Isolation of human multipotent mesenchymal stem cells from second-trimester amniotic fluid using a novel two-stage culture protocol
Human Reproduction · 2004 · Vol. 19(6) · pp. 1450–1456
Ming‐Song Tsai✉(Cathay General Hospital)
Abstract
We demonstrate that human multipotent MSCs are present in second-trimester amniotic fluid. Considering the great potential of cellular therapy using fetal stem cells and the feasibility of intrauterine fetal tissue engineering, amniotic fluid may provide an excellent alternative source for investigation of human MSCs.
Mesenchymal stem cell researchMicroRNA in disease regulationTissue Engineering and Regenerative MedicineMesenchymal stem cellAmniotic fluidAmniotic stem cellsCD90BiologyMultipotent Stem CellFlow cytometryAndrologyStem cellImmunology
MeSH terms
Amniotic FluidCell DifferentiationCell SeparationFemaleHumansPhenotypePregnancyPregnancy Trimester, SecondCell Culture TechniquesMesenchymal Stem Cells
Funding
- National Science Council
Citations
656
FWCI
7.88
field-weighted impact
References
26
Percentile
98%
vs. same field & year
Citations per year
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References
Expansion of Human Adult Stem Cells from Bone Marrow Stroma: Conditions that Maximize the Yields of Early Progenitors and Evaluate Their Quality
Stem Cells · 2002 · 960 citations
Searching for Alternative Sources of Postnatal Human Mesenchymal Stem Cells: Candidate MSC‐Like Cells from Umbilical Cord
Stem Cells · 2003 · 916 citations
Preimplantation Human Embryos and Embryonic Stem Cells Show Comparable Expression of Stage‐Specific Embryonic Antigens
Stem Cells · 2002 · 482 citations
Mesenchymal Stem Cells
Experimental Biology and Medicine · 2001 · 874 citations
Multilineage Potential of Adult Human Mesenchymal Stem Cells
Science · 1999 · 20,977 citations
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