Genetic diversity of three generations tinfoil barb Barbonymus schwanenfeldii (Bleeker 1854) from West Kalimantan, Indonesia

Abstract

The success of domestication programme and sustainability of aquaculture is highly dependent on the result of evaluation and understanding of the genetic diversity. This study is aimed to evaluate the genetic diversity of three generations, G-0, G-1, G-2 of tinfoil barb from West Kalimantan. Genetic analysis was conducted by RAPD using three primers (OPA 08, OPA 09 and OPC 02). The samples of caudal fin from each generation used 10 individuals. Formation of the population generation was done by individual selection method based superior growth. The results of intrapopulation diversity showed that the percentage polymorphism and heterozygosity of G-0 to G-2 has decreased by 30-40% with value 42.50% ; 0.163 (G-0), 27.50% ; 0.105 (G-1) and 15.00%; 0.071 (G-2). Based on the domesticated showed the genetic distance ranged from 0.26 to 0.36. The closest genetic distance was G-1 and G-2 (0.26). The domestication process that has been done can cause the reduction in genotype diversity of tinfoil barb.