Chemical composition and antibacterial activity of the essential oil from Diphasia klaineana fruits and its fractions obtained by fractional distillation

Abstract

This study aimed to analyse the chemical composition of essential oils from Diphasia klaineana fruits, its fractions and evaluate their antibacterial activity. The GC-MS analysis and agar disc diffusion and micro-broth dilution methods were carried out. A total of 43 constituents were identified in the essential oil from fruits of Diphasia klaineana (FR) and the major compounds were β-elemol (30.23%), sabinene (9.28%), guaiol (5.12%), (E)-β-ocimene (5.11%) and δ-elemene (3.53%). The FR1 and FR3 fractions collected after one hour and after three hours were rich in hydrocarbon monoterpenes and showed bactericidal and bacteriostatic activities, respectively. No activity was found with FR5, FR7 and FR9 fractions. Therefore, fractional distillation may be an interesting process to upgrade the antibacterial activity of D. klaineana fruits essential oil againt Staphylococcus aureus.