Molecular identification of Stomoxys and musca (Diptera: Muscidae) of veterinary importance in the pasture area of Ngaoundere

Abstract

Pasture flies are abundant pests in rangelands of Ngaoundere and information on their species composition is weak. For this reason a cross sectional entomological prospection using a Vavoua trap was conducted in the rainy season (September 2017) in Ngaoundere. Of a total of 568 Stomoxys (biting muscid) trapped, two species were identified 196 S. niger niger (34.51%, 9. 33 Snn/t/d) and 101 S. omega (17.78%, 4.80 so/t/d) as well as 271 non-biting Musca spp (47.71%, 12.90 m/vavoua and day). Molecular genotyping confirmed the morphological identification of Stomoxys species (S. n. niger and S. omega) and Musca species nucleotide sequences generated were 88% identical to Musca autumnalis.