Comparison of Loop mediated isothermal amplification with polymerase chain reaction for detection of methicillin resistant Staphylococcus aureus in Chevon

Abstract

Methicillin-Resistant Staphylococcus aureus has been recognized as a major nosocomial pathogen and has also been widely associated with foodborne illnesses. The main aim of the study was to compare Loop Mediated Isothermal Amplification assay and Polymerase Chain Reaction technique based on the basis of sensitivity and specificity for detection of Methicillin-Resistant Staphylococcus aureus. Total 26 Staphylococcus aureus isolates recovered from 150 raw chevon samples were used in this study. These isolates were subjected to both the techniques for detection of Methicillin-Resistant Staphylococcus aureus. In results, both the techniques could detect 2 (1.33%) isolates. The sensitivity (detection limit) of the Loop Mediated Isothermal Amplification assay was noted to be 10-fold higher than that of Polymerase Chain Reaction whereas the specificity of both was found to be similar (100%).