Effect of solvent extraction in antioxidant activity and RP-HPLC based study of phenolic acids and flavonoids and Cayratia trifolia (L.) Domin

Abstract

Cayratia trifolia (L.) Domin (Vitaceae) is perennial climber with wide array of ethnomedicinal uses. The present study was focussed to evaluate the in vitro antioxidant activity of the plant in different solvent extracts viz. 70% ethanol, methanol, chloroform and benzene, along with HPLC based identification and quantification of the phenolic acids and flavonoids. The results indicated that 70% ethanol extract was the most potent solvent for extraction of total phenolics which decreased with decreasing polarity. the phenolic content ranged between 42.200 ± 0.196 GAE/ g dry mass in 70% ethanol to 14.872 ± 0.370 GAE/ g dry mass in benzene. ABTS radical scavenging activity of 27.752 ± 0.26%/ g dry mass was observed in 70% ethanol. The HPLC analysis confirmed the presence rutin (6.077 ± 0.001 mg/ 100g dry mass), naringin (1.078 ± 0.005 mg/ 100g dry mass), syringic acid, ferulic acid and others. The present study validates the ethnomedicinal use of the plant and also indicates the need of further study designed for maximum utilization of the same in a more practical and commercial way.