DNA Barcoding of local pigs in minahasa, north sulawesi

Abstract

DNA barcoding and Molecular phylogeny reconstruction of local pigs from Minahasa North Sulawesi, Indonesia was investigated using partial sequences of Cytochrome c oxidase subunit I gene (CO1). Two location according the place of traditional pigs farm used this study that is Minahasa Utara and Minahasa was analysis the partial CO1 gene. Phylogeny trees was constructed online in NCBI site. From the results of this studied, based on partial CO1 gene, local Pigs of Minahasa cannot be sure of the position of species, because it does not have a sequence similar to the gene bank. The North Minahasa local pigs showed the similarity of the nearest CO1 sequence to the Pig DNA sequence from clone WTSI_1061-78D9. Similarly, the phylogeny construction shows the closest kinship based on the CO1 gene with Pig DNA sequence from clone WTSI_1061-78D9.