Antiradical, antimicrobial and cytotoxic activity evaluations of Alchemilla mollis (Buser) Rothm

Abstract

Alchemilla mollis (Buser) Rothm. (Rosaceae) that grows in Turkey was examined for its different biological activities. The chemical composition of 50% methanol, water and deodorized water extract were determined spectrophotometrically (total phenol, total flavonoid and total flavonols). The antiradical activity was determined by DPPH• (1,1-diphenyl- 2-picrylhydrazyl) and ABTS+• (2,2’-azinobis-3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activity assays. Antimicrobial activity was investigated by Agar Dilution Method. Finally the cytotoxic effect was evaluated on MCF 7 (human breast adenocarcinoma cell line) cell line with using Sulforhodamine B (SRB) method. The deodorized water extract showed higher antiradical activity in DPPH• method whereas water extract in ABTS+ • method. Water extract and deodorized water extract inhibited the viability of the MCF 7 cells by 70% at a concentration of 125.0 μg/mL. These results show that A. mollis may be a source of antiradical, antimicrobial and cytotoxic agent to support the medical use.