Isolation and phenotypic characterization of coagulase negative staphylococcus isolated from mastitic Cows in and around Zway town, Ethiopia

Abstract

A total of 230 Holstein freesia, Jersey and cross- breed lactating cows from farms and smallholders were examined during the study period and 97 (42.2%) cows were positive for mastitis by CMT screening and clinical examination of udder. out of these cows 7.0% (16/230) and 35.2% (81/230) showed clinical and subclinical mastitis respectively. Blind teat was examined in 12(1.3%) out of 920 quarters. Multivariable logistic regression showed that there was a statistically significant difference (P 0.05). The association of the different potential risk factors and the occurrence of mastitis in and around Zway/Batu farms and smallholder dairy cows of Zway area. Accordingly, the risk factors such as breed (p=0.004, X2 11.23), lactation stage (p=0.000, X2= 26.63), floor type (p=17.48, X2=0.000), milk hygiene (p=21.73, X2=0.000), parity (p=29.07, x2=0.000), age (p=29.07, x2=0.000), showed statistically significant with the occurrence of mastitis. Whereas antibiotic used (p=7.59, X2=0.06) which is statistically insignificant. Antibiotic susceptibility to Amoxicillin, Naldic acid, Oxytetracycline, Cefoxitin, Streptomycin, Compound sulphonamide, Kanamycin and Ceftriaxone was determined by agar disc diffusion (Kirby- Bauer method). All the current isolates were sensitive to kanamycin, Amoxicillin and Ceftriaxone; moderately sensitive to streptomycin and Oxytetracycline. However, the isolates were resistant to compound sulphonamide, Naldic acid and cefoxitin which is similar to different researches made on similar isolates. Since mastitis is an economically important disease, hygienic milking practice, use of effective antibiotics, proper extension packages to dairy farm owners and strategic mastitis control programs should be of paramount importance.