Development of spectroscopic method for quantification of starch in milk

Abstract

A spectroscopic technique was utilized to develop method for quantification of starch in milk. For application of spectroscopy, colour was developed by reaction between starch and iodine, absorption of the colour was measured at 549 nm and standard curve was constructed for concentration of starch versus absorbance obtained. Recovery factor was developed from amount of starch spiked and amount of starch obtained from standard curve. After developing the method, it was validated for performance by true (known) sample approach. The concentration of starch obtained by application of the developed method was compared with the concentration of the starch actually added for preparation of the sample. For statistical analysis t-test: two sample assuming equal variances was applied. The validation of method developed for quantification of starch suggested that the difference between the estimated amount of starch and amount of starch actually added to milk was statistically non-significant.