Standardization of ginger callus culture and its comparison with ginger rhizome and callus treated with extrinsic molecule on their antioxidant activity

Abstract

Zingiber officinale as a medicinal plant exhibits good antioxidant properties. In this study, the ginger leaves and ginger rhizome were selected as explants for the callus induction and plantlet production and were cultured on MS medium supplemented with various growth regulators. In addition to the induced callus, elicitors such as Salicylic acid, Yeast extract and Glycine were used as extrinsic molecules to check for the enhancement of antioxidants and phytochemicals compared to the callus and rhizome produced cultures without elicitors The highest significant amount of antioxidant was (45% to 50%) recorded by DPPH assay. This proved the effect of the elicitation of ginger cultured tissues in the antioxidant study that would be of immense importance for pharmacological, cosmetic and agronomic industries in the future as induction of callus reduces time.