Callus cell weight, antioxidant, carbohydrate, pigment and nutritional properties from broccoli explants in vitro: A nutritional vegetable

Abstract

The purpose of the study was to evaluate the callus cell weight, antioxidant, carbohydrate, pigment and nutritional properties from broccoli explants as nutritious vegetable. The results showed that the higher callus weight was found in the cultured leaf cutting than root and shoot tips cutting in the concentration of IBA 1.0 + 2.0 mg/l BAP, IBA 2.0 + 2.0 mg/l BAP, and IBA 3.0 + 2.0 mg/l BAP combination. The highest callus weight was found in the cultured leaf cutting than root and shoot tips cutting at the concentration of IBA 2.0 + 2.0 mg/l BAP. Carbohydrate (as inverted sugar and glucose), chlorophyll and nutrient content (K+, NO3-, Ca++ and Na+), total phenol, antioxidant (DPPH), flavonoid and total antioxidant were found in the concentration of IBA 2.0 + 2.0 mg/l BAP combination from broccoli leaf cutting. The results seemed that it was better for nutrition and growth to use the combination of the IBA and BAP in the concentration of 1.0-3.0 mg/l to regenerate root, shoot, leaf and callus cell proliferation and nutrition of broccoli from root tip, shoot tip and leaf cutting.