In vitro analysis of 1, 8-cineole in the plasma of domestic fowl by Gas Chromatography–Mass Spectrometry

Abstract

1,8-cineole is a major monoterpenoid found principally in essential oils of Eucalyptus globulus, Eucalyptus polybractea, Helichrysum gymnocephalum, Zingiber chrysanthum and. Rosmarinus officinalis. It is considered safe and has been used as a flavoring agent in food products, fragrances, cosmetics and phytogenic feed additives (PFAs) in animal health care and livestock production. The present study was undertaken to standardize the protocol for recovery of the phytobiotic 1,8- cineole from the blood of fowl. The parameters were optimized for determination of 1,8-cineole by GC-MS using a single quadruple GC-MS instrument (GCMS-QP2010 Ultra, Shimadzu, Japan) coupled with an auto sampler and Teledyne Tekmar Versa. 1. The retention time was 10.2 min on scan mode and the limit of detection (LOD) of 1,8-cineole was 0.001 μg/mL on SIM mode. Maximum recovery from was obtained with hexane as the solvent and the recovery percentages from plasma fortified with 0.25, 0.5 and 1 ppm was 81.76, 84.23 and 82.13 respectively.