In vitro antidiabetic and antioxidant activities of lupeol isolated from the methanolic extract of Andrographis echioides Leaves

Abstract

In the present study the lupeol isolated from the methanolic extract of leaves of Andrographis echioides was studied for alpha amylase and alpha glucosidase inhibition using an in vitro model. The isolated compound lupeol was also examined for its antioxidant activities by using free radical 1, 1-diphenyl-2-picryl hydrazyl (DPPH) scavenging method. The study revealed that the different concentration of lupeol exhibit potent radical scavenging activity using DPPH as substrate. The lupeol exhibited significant α-amylase and α-glucosidase inhibitory activities with an IC50 value 36.2±0.42 and 41.4±0.34 % respectively and well compared with standard acarbose drug. Therefore, it is suggested that lupeol is a potential source for natural antidiabetic and antioxidant compounds and could have potential use in the management of diabetes mellitus.