Development and validation of RP-HPLC and HPTLC method for the estimation of curcumin in haridrakhand polyherbal tablet formulation

Abstract

Haridrakhand is polyherbomineral Ayurvedic formulation employed in skin disorders, allergies, shitapitta, urticaria, and psoriasis. A simple, specific, precise RP-HPLC and HPTLC method for estimation of Curcumin in Haridrakhand tablet formulations were developed and validated as per ICH guidelines Q2(R1). The development was done using precoated TLC plate’s lino mat IV sample spotter. Toluene: ethyl acetate: methanol (4: 5: 1 % v / v / v) were used as mobile phase. Measurements were performed at 421 nm in using Server vision cats-server. The developed HPLC method involved use of C18 ODS, (250× 4.6) mm, 5 μ column. Acetonitrile: 0.05% orthophosphoric acid (70:30) was used as mobile phase at 0.7 mL/min flow rate and 394 nm wavelength maxima for detection. The linearity range 50-1000 ng/spot was set for HPTLC and for HPLC 1-25 µg/ml. Rf value and retention time by was found to be 0.421±0.03 and 8.387 by HPTLC and HPLC respectively.