Validated stability indicating method for Karanjin using HPTLC and HPLC

Abstract

Karanjin is an active marker present in Karanja seed. Seed powder is valued for Anti-Plasmodia activity, Anti-Inflammatory activity, Anti-ulcer Activity, Hypoglycemic and Hypolipidemic Activity, Antiviral Activity, Ant diabetic activity, renal protective activity. The present study was aimed to develop and validate stability indicating method for karanjin by HPLC and HPTLC. The development was done using TLC plates precoated with silica gel 60F254. Toluene-Ethyl acetate (8:2 v/v) were used as mobile phase and scanning was done by using TLC Scanner III. The developed HPLC method involved use of RP-C18 (150 × 4.6 mm) column. The detection done at 260 nm using PDA detector having mobile phase Methanol: ACN: Water (70:15:15 v/v/v). The flow rate was kept as 1 mL/min. The linearity range 80-400 ng/band was set for HPTLC and for HPLC 10-50 µg/ml. Stress degradation studies were carried out for karanjin as per the ICH guidelines. The characterisation of stress sample were done using LCMS.