Standardization of DNA extraction protocols from leaves of underexploited fruit crop carambola (Averrhoa carambola L.)

Abstract

The objective of the study was to recommend the cheap, efficient time and genomic DNA extraction method for the underexploited fruit crop carambola (Averrhoa carambola L.). Based on the quality of genomic DNA determined by the ratio of A260/A280 and the amplifiable quality of DNA determined by the horizontal agarose gel electrophoresis using 1% agarose in TBE buffer at constant voltage of 60V, the modified CTAB methods yielded good quality DNA having OD (260/280) ratio of 1.52 having concentration of DNA 0.94 (µg/µl) as compared to Doyle & Doyle method OD (260/280) ratio of 1.45 having DNA concentration 0.68 (µg/µl) and the time requirement for this two DNA methods are almost same. Therefore, CTAB method could be recommended for the efficient DNA extraction method for this minor fruit crop for exploitation of superior genotype using molecular marker in the future.