Rapid and efficient method of genomic dna extraction from sweet sorghum [<em>Sorghum bicolor</em> (L.)] using leaf tissue

Abstract

Extraction of genomic DNA from plant leaf tissue is a basic requirement for molecular biology experiment as well as in plant breeding for crop improvement. The purity of DNA is affected by varying level of polyphenols, polysaccharides and secondary metabolites with are associated with DNA at the time of extraction. A variety of DNA extraction methods and kits are available, however they are costly, low yield and time consuming. Here, we describe a simple and efficient method of DNA extraction from sorghum leaf. The present study is based on conventional cetyl trimethyl ammonium bromide (CTAB) method with four major modifications to isolate genomic DNA from seven different accessions of sorghum and the yield of DNA was varied from1550.2 ngµl-1 to 1910.5ngµl-1 in 100 µl final volume of TE buffer. The purity of DNA sample was found to be varied from 1.53 to 2.16 based on the absorbance at A260 and A280 ratio. This method is very simple as it not requires liquid nitrogen or magnetic beads to grind the leaf sample which is mostly unavailable to some undergraduate laboratories.