Effect of different 2,4-D concentration on callus induction of sugarcane varieties

Abstract

An efficient protocol for callus induction and response of callus to different concentrations of 2,4-D of two sugarcane varieties CoN 13073 and CoN 09072 has been developed and reported here. Leaf whorl and meristem taken as an explant portions for callus induction in both the varieties. % The results showed that leaf whorl was found suitable for quick callus induction in both the varieties. Both the explant portions exhibited differential performance towards 2,4-D concentrations for callus induction. Earlier callus induction was observed in variety CoN 13073 where leaf whorl was an explant portion. At 0 level of 2,4-D concentration poor and moderate callus induction was observed in both the varieties. At 3 mg/l 2,4-D concentration, profuse and good quality of callus was observed in both leaf whorl and meristem, with the increase in concentration of 2,4-D above 4 mg/l quantity and quality of callus was reduced among the varieties.