Effect of different concentrations of 2, 4-dichlorophenoxy acetic acid on callus induction of sugarcane (Saccharum officinarum l.) Var. Co 86032 and com 0265

Abstract

Standardization of protocol for callus induction and response of callus to different concentrations of 2, 4-D of two sugarcane genotypes (Co 86032, CoM 0265) was established through callus culture using leaf whorl and meristem as an explant. The results showed that meristem as well as leaf whorl explants were suitable for callus induction in sugarcane. MS medium supplemented with 3 mg/l and 4 mg/l of 2, 4-D was found most suitable for callus induction and multiplication in both the varieties. This treatment also took minimum number of days for callus induction and maximum callus induction percentage in both the genotypes. Profuse and good quality callus induction was observed in MS medium + 3 mg/l and 4 mg/l 2, 4-D in both the explants genotypes.