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Effect of Cysteamine, Epidermal Growth Factor (EGF) and Estrous Cow Serum (ECS) on In-vitro Maturation of Bovine Oocytes
International Journal of Chemical Studies · 2018 · Vol. 6(1) · pp. 1444–1447
Abstract
A total of 526 good quality bovine oocytes were subjected to in-vitro maturation of oocytes at 38.5 0C in humidified atmosphere of 5% CO2 tension for 24 hours and matured in-vitro in medium-I or control (TCM-199+10% FBS+L-glutamine+sodium pyruvate+Gentamicin+pFSH+ Estradiol 17-β), medium-II (control+5% ECS), medium-III (control+100µM/ml cysteamine), medium-IV (control+10ng/ml EGF) and medium-V (control+ 5% ECS+ 100µM/ml cysteamine+10ng/ml EGF). The rates of maturation based on cumulus cell expansion and nuclear maturation varied significantly (p
Reproductive Biology and FertilityOvarian function and disordersSperm and Testicular FunctionCysteamineSodium pyruvateIn vitro maturationIn vitroEpidermal growth factorChemistryAndrologyGlutamineBovine serum albuminEndocrinology
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