Effect of antioxidant on in vitro maturation of bovine follicular oocytes

Abstract

The present study was conducted to assess the effect of α-tocopherol on in-vitro maturation of bovine follicular oocytes. The aspirated follicular oocytes were in-vitro cultured in TCM-199 medium at 38.5oC with 5% CO2 for 24 hours in a CO2 incubator. The medium TCM-199 supplemented with follicular fluid, oestradiol-17β, p-FSH, gentamicin, sodium pyruvate, cysteamine and fetal bovine serum was used as control. In the treatment groups, the control medium was supplemented with 100 µM and 150 µM α-tocopherol. The rates of cumulus cell expansion recorded in 100 µM, 150 µM α-tocopherol supplemented and the control group of oocytes were 74.28 ± 1.66, 73.59 ± 0.78 and 74.46 ± 2.68 per cent and polar body formation were 50.06 ± 2.40, 45.43 ± 4.20 and 44.02 ± 4.37 per cent, respectively.