Paternal contribution in the Intra-specific hybrid of Grewia optiva as reveled by paternity analysis using molecular markers

Abstract

Superior genotype of Grewia optiva, SO-2 (Nauni, Solan) as female and BI-4 (Khutira, Bilaspur) as male genotype were selected on the basis of their morphological and genetic variability, the plants were hybridized to produce an intra-specific hybrid (F1). The F1 obtained is morphologically similar to its paternal parent to confirm its parentage, fourteen RAPD and eleven ISSR markers were used in the study. The primers used were successful in confirming hybridity. The hybrid was highly similar to their male parents. Similarity of hybrid between the combined banding of genotypes for individual primers varied from 17 to 100 % (RAPD) and 33 to 80 % (ISSR). Whereas, overall similarity of hybrid was 71 % for RAPD and 68 % in ISSR. In the present study, RAPD and ISSR profile generated by individual primers exhibited up to 100 % and 80% polymorphism respectively. From the present investigation, we can conclude that the intra-specific hybrid (F1) produced is a cross between SO-2 and BI-4 and it is evident from both morphological character and molecular phylogeny using RAPD and ISSR profiling the F1 is tends to be more similar to its paternal parent (BI-4) than that of maternal (SO-2). So, RAPD and ISSR profiling can be successfully used for genetic fingerprinting including verification of intra-specific hybrid in G. optiva.