Analysis of Pimpinella anisum (Yansoon) seeds using gas chromatography mass spectrometry

Abstract

The extraction of milled Pimpinella anisum (Yansoon) seeds were performed using two different extraction methods; organic extraction with methanol (polar), hexane (nonpolar) and hydrodistillation method for separation essential oil from Anise seeds. Analysis of Pimpinella anisum (Yansoon) seed using Gas Chromatography – Electron Ionization / Mass Spectrometry (GC-EI/MS) technique was applied with full scan mode within mass range from 12 – 500 mass to charge ratio (m/z). The chemical composition was identified for trace level of 0.01% using Agilent Mass Hunter unknown analysis software and NIST14 library of mass spectrums. The hydrodistillation extract and hexane extract of essential oil were revealed the highest abundance for trans-Anethole with 96.54% and 72.15% respectively, while methanolic extract was given the highest abundance of trans-Pseudoisoeugenyl 2-methylbutyrate and Butanoic acid, 2-methyl-, 4-methoxy-2-(3-methyloxiranyl) phenyl ester 58.93% and 28.93% respectively. The yield of Anise seed oil was 2.5 – 3.0%.