In vitro biomechanical property of porcine Acellular diaphragm matrix (ADM) used for surgical reconstruction in abdominal defects

Abstract

The biological materials used in reconstructive surgery harvested from other animal required chemical or enzymatic treatment to remove the cellular components in order to minimize its antigenicity. In the present study, sodium deoxycholate treated decellularized Porcine Acellular Diaphragm Matrix (ADM) tested for its biomechanical properties compare to native fresh porcine diaphragm in an Instrons Universal Tensile Machine (UTM). Four different sizes of ADM and Fresh Diaphragm (5, 10,20 and 40 mm) tested in the machine keeping constant gauze length 50mm. Prepared Porcine Acellular Diaphragm Matrix (ADM) retained sufficiently high tensile strength (93.94%) and elongation percentage (98.44%) when compared with fresh unprocessed diaphragm.