Use of the bubaline acellular diaphragm matrix (ADM) for repair of abdominal wall defects in four different species of animals

Abstract

Decellularization of xenogenic biomaterials has been highly desired for implantation without considerable adverse inflammatory and immune responses. The present study was undertaken to decellularize the diaphragm of buffalo origin and to evaluate the efficacy of this acellular diaphragm matrix (ADM) for the repair of abdominal wall defects. Buffalo diaphragm was decellularized with 1% sodium deoxycholate (SDS) for 48 h. Microscopic examination of the detergent treated matrix confirmed complete extraction of stromal cells and orderly arranged collagen fibres. Prepared ADM scaffolds were used for repair of abdominal wall defects in four different species of animals. The abdominal wall defects repaired with ADM remained sound over a period of 3 months. All the defects repaired with ADM healed completely without graft rejections. The present study suggested that ADM may be used safely for repair of abdominal wall defects in different species of animals.