Occurrence and characterization of Xanthomonas axonopodis pv. phaseoli associated with seeds of mungbean (Vigna radiata L. Wilczek) grown in Rajasthan state, India

Abstract

In the two successive major and various minor field visits, a total of 151 seed samples were collected from 14 districts of Rajasthan state, India which is the major producer of mungbean in the country and screened for association of Xanthomonas axonopodis pv. phaseoli with seeds of mungbean. Total 146 seed samples were found to be infested with this pathogen and showed its high association with mungbean seeds. The seeds were categorised on the basis of external symptoms such as discoloration of seed coat and hilum. The pathogen was isolated from seeds by direct plating on NA and confirmed on differential and semi-selective medium viz. MT and XCP1. It was also confirmed by biochemical test, pathogenicity test on host plant and sequencing of amplified 16srRNA product.