Seed protein profiling of rice genotypes

Abstract

Biochemical characterization and quantification of seed protein of a diversified group of 117 rice genotypes consist of land races, wild rice, advanced breeding lines and popular varieties of Chhattisgarh India was done in the present study. Total fifteen bands were observed in which eight were polymorphic. Total seed protein was quantified by Bradford methods and on the basis of protein percent the group was divided in three categories less medium and high three cultivars i.e. Badagadakhurta (8.27%) followed by Kalokuchi (8.05%) and Nunki Gurmatia (7.20%) among 117 showed highest seed protein percent. Similarity coefficients varies from 65 to 100% based on jaccard’s the genotypes were grouped into six clusters and shows Cluster I and II (d) had 5 genotypes present, cluster I had two subgroup Kalamkati and G-21 similar and Rajeshwari, IR 87728-367-B-B and Uri are some differences between genotypes similarity coefficient of this cluster 55% while cluster II (a) 15 genotypes and three subgroup cluster II (a) Moroberekan genetically different to the other. clusters II (c) two, cluster II (e) divide into two subgroup and present 29 genotypes, and cluster II (b), the largest one with 114 genotypes this cluster three genotypes Poornima, Basmati, and E 2526 are different diversity than other. Overall on the cluster Rajeshwari, IR-87728-267 and Uri genotypes was found to be different. Thus, the It is also most likely that the protein marker would then be able to estimate the amount of genetic diversity in the genus and perhaps obtain an indication as to the origin of the rice genotypes. On the basis of cluster analysis the genotypes, Rajeshwari, IR-87728-267 and Uri for protein marker and Badagadakhurta and ARC 10376 for protein content seems to be different and further would be used to enhancement of nutritional quality of rice.