Analyzing the genetic variability of rice weevil, Sitophilus oryzae (Coleoptra; Curculionidae) in different geographic locations of district Charsadda 2018, Khyber Pakhtunkhwa (KP), Pakistan

Abstract

The rice weevil (Sitophilus oryzae) is a grain feeding specie belonging to family Curculionidae, order Coleoptra. S. oryzae is ranked as the first important pest causing the highest damage of 50 tested countries. The present research work was focused on the molecular and genetic analysis of S. oryzae from district Charsadda Khyber Pakhtunkhwa (KP). The Sieve method was randomly used to capture the adult S. oryzae. To separate the red flour beetle from wheat flour mills we used sieves with 4mm mesh. DNA extraction was made from individual adult S. oryzae using TNE salt extraction protocol. RAPD markers were used for PCR amplification during the first step to find out genetic variability, gene flow and rate of migration in different S. oryzae populations. The PCR products were analyzed using gel electrophoresis. The data generated through RAPD markers were analyzed through POPGENE software. The current study can be taken as a starting point for future researchers aimed at defining the level of intra and inter species genetic diversity. Based on the results, it is suggested that polymorphic markers out of RAPD method be used for understanding genetic variability in S. oryzae population in Pakistan and could be helpful in future vector control programs.