Isolation, morphological identification and In Vitro antibacterial activity of endophytic bacteria isolated from Ocimum sanctum (Tulsi) leaves

Abstract

Fresh leaves of Ocimum sanctum (Tulsi) was procured and divided into five samples and each sample was again divided into 5 sub samples and separated for isolation of endophytic bacteria. Characterization of bacteria was done according to its morphology by grams staining. The antibacterial effect of endophytic bacteria was studied against three gram positive and gram negative bacteria by disc diffusion method with known antibiotic ciprofloxacin (Ci) as standard. Most of the samples on kings B media depicted irregular shape, flat elevation, undulated, rough, opaque and white in colour. Most of the samples on blood agar showed irregular, raise elevation, undulated, smooth, opaque and all isolates were non haemolytic and non-chromogenic. The growth of all isolates in BHI broth showed turbidity. The microscopic examination revealed that maximum isolates were gram positive and rod shaped. Good antibacterial activity was observed against Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli and Salmonella Typhimurium.