Effect of different fungicides and bio agents against Colletotrichum truncatum (Schw.) causing anthracnose of greengram [Vigna radiata (L.) Wilczek] in vitro

Abstract

A study has been conducted at Department of Plant Pathology, University of Agricultural Sciences Raichur on evaluation of new formulations of fungicides molecules and bioagents against Colletotrichum truncatum, causing anthracnose of greengram. Seven systemic, seven contact and seven combi fungicides were evaluated against C. truncatum under in vitro by poison food technique. Ten strains of fungal bioagents (Trichoderma viride and T. harzianum) and nine strains bacterial bioagents (Bacillus subtilis and Pseudomonas fluroscens) were tested against Colletotrichum truncatum under in vitro by dual culture technique. Among the contact fungicides evaluated, propineb 70% WP was recorded highest mean mycelial inhibition (82.71%) followed by copper oxy chloride 50% WP (78.51%). Among the systemic fungicides evaluated, thiophanate methyl and triazole group of fungicides like propiconazole 25% EC, difenconazole 25% EC and tebuconazole 25.9% EC recorded highest mycelial inhibition of C. truncatum at all the three concentrations (0.05, 0.1 and 0.15%) and azoxystrobin showed least mean mycelial inhibition (75.55%). Among the combi fungicides evaluated, cent per cent inhibition was recorded in tricyclozole 18% + mancozeb 62%WP and carbendazim 12% + mancozeb 63% WP at 0.3 per cent concentration. Among the ten fungal bioagents screened against C. truncatum, the highest mycelial inhibition was found in the T. viride strain (Tv- 29) followed by strain Tv– 1 and Tv– 10. While among the bacterial bioagents, B. subtilis strain (Bs – 21) and P. fluorescens strain (Pf – 26) showed highest mycelial inhibition.