A comparative study on the in vitro effect of treatment time of ems on secondary metabolite production in Andrographis paniculata (BURM.F.) Nees

Abstract

Effect of 0.01% of EMS on secondary metabolite production in Andrographis paniculata was conducted in this study. Calli were derived from leaf explants inoculated on Murashige and Skoog’s medium supplemented with 2 mg/l NAA, 1 mg/l kinetin and 50 mg/l phenylalanine. In 0.01% EMS treatment for 5 h resulted in a maximum amount of fresh weight (1.55±0.06 g) and dry weight (0.12± 0.01g) of callus and also the callus response (73.33). Treatment of 0.01% EMS also resulted in 6 - fold increase in andrographolide production when compared to control. The study also showed that concentration and treatment time of EMS influenced the secondary metabolite production in the treated samples.