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In vitro studies on the effect of Sodium azide treatment on secondary metabolites production in Andrographis paniculata (Burm. f.) Nees

Journal of Pharmacognosy and Phytochemistry · 2018 · Vol. 7(5) · pp. 2411–2415

Abstract

Effects of 0.01% SA (Sodium azide) for 3 hours on secondary metabolites production in Andrographis paniculata was carried out in this study. Calli produced from leaf explants inoculated on Murashige and Skoog’s (MS) medium supplemented with 2 mg/l NAA, 1 mg/l Kinetin and 50 mg/l Phenylalanine. Treatment of 0.01% SA for 3 hours positively influence in secondary metabolites production when compared to the control. The study also showed that 0.01% SA for 3 hours treated samples showed enhanced production in the fresh weight, dry weight of calli and callus induction frequency compared to the control.

Andrographolide Research and ApplicationsPlant tissue culture and regenerationPlant Genetic and Mutation StudiesAndrographis paniculataKinetinSodium azideCallusExplant cultureChemistryInoculationHorticultureSecondary metaboliteIn vitro
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In vitro studies on the effect of Sodium azide treatment on secondary metabolites production in Andrographis paniculata (Burm. f.) Nees · Scinovex