Genetic variability analysis in sugarcane (Saccharum spp. complex) through mutagenesis

Abstract

Two sugarcane varieties CoN-05071 and CoN-07072 was used for induction of genetic variability through in vitro mutagenesis. In this study 25-30 days old white globular Callus tissue is considered as suitable explants material. To undergo mutagenic treatments with two levels of EMS 0.3% and 0.2% for 30min and 60min and resulted very good callus appearance in both the varieties. Maximum callus survival percent was found in callus treated with EMS 0.3%+30min (90.77%) in CoN-05071 whereas, EMS 0.2%+30min callus treated was showed maximum survival percent (78.63%) in CoN-07072. Maximum regeneration percent was found in explants treated with EMS 0.3%+30min (78.77%), and SA 0.2%+30min (82.45%) in CoN-05071 and CoN-07072 respectively. In the present study EMS 0.2% and 0.3% and SA 0.2%, 0.3% treatments were most effective mutagenic treatments. The effectiveness decreased with increasing time intervals.