High frequency in vitro callus induction in potato (Solanum tuberosum L.) Genotypes

Abstract

The present study was conducted to evaluate the effects of 2, 4-D on in vitro callus induction in two potato genotypes i.e. Kufri Bahar and Kufri Surya. Best callus induction with slightly lower contamination percent, number of days required for callusing and higher callus weights from leaf segments was reported in Kufri Bahar on full-strength basal MS media supplemented with 10.0 mg/L 2,4-D. The increasing concentrations of 2, 4-D exhibited an increase in callus induction percentage in both studied genotypes, but at the higher concentrations callus induction percentage reduced drastically. The protocol thus developed may be efficiently used to induce high frequency callus in potato genotypes for generating somaclonal variants possessing agronomically desirable traits.