Phytochemical and antioxidant activity of methanolic leaf extract of Alstonia scholaris Linn.

Abstract

Alstonia scholaris is a plant of the Apocynaceae family and as great medicinal important. It is widely used by triable people to treat various for the treatment of diseases and ailments such as antimicrobial, anticancer, anti-inflammatory, anti-infertility and wound healing activities. The Alstonia scholaris leaves was collected wash with saline water, shade dried room temperature for 20 days. The dried plant materials was pulverized into fine powder using a grinder (mixer). About 9gm leaf powder was extracted in soxhlet apparatus with 150ml methanol. Crude extract obtained is subjected to column chromatography for purification of active compounds. The individual fractions were subjected to qualitative the identification of the active compounds. Methanol extract and toluene : chloroform (80:20) revealed the presence of the presence of alkaloids, flavonoids, carbohydrate, phenolos, steroids, saponins and tannins, amino acids, terphenoids the quantification of total alkaloids, total phenols, total flavanoids, and total tannin has been identified leaves of crude extract and column purified samples by spectrophometer. The present study identify with standardization of the plant leaves of various phytoconstituents and antioxidant potential with column purified compounds were free radical scavenging assay of 2,2 –diphenyl-1-picryl-hydrayl (DPPH),SO & ABTS in in vitro assay. The present study revealed methanol leaf extract of Alstonia scholaris would exert several benificial effects by virtue antioxidant activity and need further investigations for it to be use in clinics in the future.