Biochemical studies and anti-nutritional factors of lentil (Lens culinaris medik) varieties

Abstract

The present research work entitled “Biochemical studies and anti-nutritional factors of lentil (Lens culinaris Medik) varieties”was conducted during Rabi season 2016-17 at the Agronomy research farm and laboratory of Agriculture Biochemistry Narendra Deva University of Agriculture & Technology, Kumarganj Faizabad (UP). Was adopted with three replications. Following lentil varieties were grown with proper agronomic practices and the seeds of ten varieties of Lentil namely NDL-1 (C) HUL-57 K-75 DPL-15 NDL-2 IPL-325 VL-148 NDL-22 PL-192 NDL-15 were undertaken to lentil varieties with successive were executed in Completely Randomized Design (CRD) was adopted with three replications. Following lentil varieties were grown with proper agronomic practices and the seeds of ten varieties were collected after harvesting and use for analysis of biochemical Parameters. viz Total chlorophyll content peroxidase enzyme). Were catalase activity. Tannin content and Phytic acid content. The data obtained in the experiment showed the highest total chlorophyll content at 30, 60, 90 and 120 days intervals in lentil varieties was recorded in the range of 0.40 to 0.48 mg/g, 0.35 to 0.45 mg/g, 0.26 to 0.40 mg/g and 0.10 to 0.19 mg/g. The data pertaining to the activity of catalase enzymes at 30, 60, 90 and 120 days in leaves of lentil varieties was found in the range of 36.07 to 39.87 Unit/g FW, 56.29 to 59.77 Unit/g FW, 67.97 to 70.81 Unit/g FW and 35.34 to 45.81 Unit/g FW NDL-1 variety. The activity of peroxidase enzymes at 30, 60, 90 and 120 days in leaves of lentil varieties was obtained in the range of 67.14 to 71.82 Unit/g FW, 87.27 to 91.92 Unit/g FW, 106.15 to 109.32 Unit/g FW and 78.54 to 81.34 Unit/g FW All the varieties were found significant. Highest tannin content was reported in the varieties IPL-325 (0.97 mg/g).and Maximum phytic acid content was recorded in the varieties IPL-325 (2.13 mg/g).