Mutagenic effect of gamma rays, EMS, NG and their combinations for induction of chlorophyll and macro-mutations in mungbean (Vigna radiata (L.) Wilczek)

Abstract

Seed treatment of two mungbean genotypes, BKG-1 and OUM 11-5, with gamma rays, EMS, NG and their combinations induced wide spectrum of chlorophyll mutations (Albina, xantha, chlorina, viridis and sectorial) and morphological macro-mutations in M2. Chlorina types were more frequent in both the genotypes followed by xantha and albino. Among morphological mutations, quadrifoliate, dwarf plant, lobed leaf and pentafoliate mutants induced at high frequency in both the genotypes. NG in single and combination treatments induced higher chlorophyll and morphological mutations than EMS and GR in both the genotypes. The frequency of chlorophyll, morphological and total macro-mutations showed a dose dependent increase in both single and combined treatments in case Gamma-rays and EMS in both the genotypes. While, morphological macro-mutation frequency was highest in 0.010% NG among single treatments; and 0.4% EMS + 0.010% NG in BKG-1 and 400 Gy gamma rays + 0.010% NG in OUM 11-5 among combination treatments in both the genotypes.