Macro-mutations induced by EMS, gamma-rays and their combined treatments in Chickpea (Cicer arietinum L.)

Abstract

Treatment with EMS (0.05%, 0.10% and 0.15%), gamma-rays (150 Gy, 200 Gy, 300 Gy) and their all possible combinations were utilised to generate genetic variability in chickpea variety HPG-17. The potential of each treatment was analysed on the basis of variability generated which included range of viable and non-viable mutants. Treatment of 0.10% EMS produced maximum viable macro-mutants. Low doses of both EMS and gamma rays individually, as well as in combination failed to produce chlorophyll mutants. Highest frequency of chlorophyll mutants was observed in treatment 300 Gy + 0.15% EMS. Wide range of mutants for plant type, leaf colour, leaf shape, seed colour, seed texture and sterility was also observed.