Development and validation of HPLC methods for the standardization of Stigmasterol & Lupeol from the extract of Butea monosperma (Lam) and it’s formulation

Abstract

The determination of natural compounds Lupeol and Stigmastrol in plant extracts using HPLC Shimadzu model Lc20AD AHT to manual sampler (UV-SPD-20A Detector) is reported. The methods were applied to the analysis of Lupeol and Stigmastrol in petroleum ether extract originating of Butea monosperma (Lam) bark. The method was validated using ICH guidelines in terms of precision, repeatability, recovery and accuracy. Regarding HPLC method validation, the optimize mobile phase system used was Methanol: Water (98:2%v/v) with the 1ml/min flow rate and the detection wavelength was 220nm.