In vitro screening of the biological activity of combined extracts of two medicinal plants and their standardization by validated analytical methods using standard markers

Abstract

The aim of the present study is to carry out in vitro antiarthritic and antigout activity of the combined extracts of two plants namely Mangifera indica and Hygrophila spinosa and their standardization by HPTLC and HPLC methods using appropriate markers. The synergistic properties of the extracts pertaining to antiarthritic and Antigout activities were evaluated. The mobile phase for HPTLC was optimized as toluene: ethyl acetate: methanol, 15:3:1.5%v/v/v. The presence of mangiferin was detected at 270nm under UV light and the lupeol was detected after derivatization with Liebermann burchard reagent at 366nm. In the HPLC method, the optimized solvent system used was acetonitrile: methanol: 20mM potassium di hydrogen phosphate 30:20:45% v/v/v and detection was made at 210nm. The combined extracts produced better activity than the individual extracts of the respective plants. Hence, these combined plant extracts were standardized, quantified and validated by newly developed HPTLC and HPLC methods as per ICH guidelines.