In-vitro anthelmintic activity of Citrus limon leaf and its phytochemical investigation

Abstract

The crude extract of Citrus limon L was evaluated for anthelmintic activity using adult earthworm Eisenia Foetida. The prevalence of worm infestations is high in underdeveloped countries due to poor sanitation and lack of health education. Citrus limon L. leaf belonging to family Rutaceae is a common plant, native to Asia. Aqueous extract of Citrus Limon L leaf was subjected for the preliminary phytochemical analysis and found the presence of alkaloids, tannins, phenolic compounds, carbohydrates, proteins, oils, fats and flavonoids. Anthelmintic activity was performed in 3 different concentrations such as 5mg/ml, 10mg/ml and 20mg/ml. The highest concentration showed the maximum activity. All the extracts exhibited better activity than standard compound Albendazole.