Biochemical composition and storage protein profiling of mungbean (Vigna radiata L. wilczek) cultivars

Abstract

The experimental trial on 12 mungbean cultivars were conducted and the findings of the pooled data of two years revealed that biochemical composition viz. moisture, total carbohydrate, crude protein, soluble protein, methionine, crude fat, calorific value, total phenol and ash content varied highly significantly across the tested cultivars and ranged from 8.74 to 9.96%, 61.18 to 63.47%, 23.99 to 26.15%, 15.83 to 19.72%, 0.79 to 1.76 g/16gN, 1.12 to 1.62%, 347.93 to 360.19 kcal/100g, 62.35 to 89.61mg/100g and 3.42 to 4.01% with an overall mean of 9.61%, 62.55%, 25.08%, 17.97%, 1.26g/16gN, 1.37%, 353.65kcal/100g, 73.04mg/100g and 3.67%, respectively. SDS-PAGE of seed storage proteins of 12 cultivars of mungbean led to detection of 26 polypeptide bands with molecular weights of the resolved peptides ranged from 104 kDa to 18 kDa. Dendrogram based on electrophoretic data grouped the 12 cultivars into two major clusters, cluster I and cluster II.In the present study, a peptide band of 18 kDa was detected on SDS-PAGE that may be 11 S globulin subunit, peptides with molecular weights 28 kDa was also detected on SDS-PAGE that may be basic 7S subunit, while peptide with molecular weight 23kDa may be 11S globulin and peptide with molecular weight 32 kDa and 25 kDa peptide might be 8S vicilin subunit according to earlier reports.