Plantlet regeneration of naga king chilli (Capsicum Chinense JACQ.) from nodal segments through in vitro technique

Abstract

The present investigation was undertaken at the Department of Horticulture, Nagaland University to standardize the protocol for plantlet regeneration of Naga King Chilli (Capsicum chinense Jacq.) by using nodal segments as explants. Nodal segments (1.0 cm) were cultured on MS medium supplemented with either BAP or KIN @ 0-8 mg L-1 respectively for shoot proliferation, alone and in combination with 0.5 mg L-1 IAA. The medium containing 8 mg L-1 BAP + 0.5 mg L-1 IAA recorded the maximum response (88.88%) for shoot proliferation. The highest shoots (5.49) with maximum bud length (1.9 cm) were proliferated during the third subculture. Regenerated shoots were maintained in MS basal medium for further elongation thereafter, transferred to MS medium supplemented with auxins viz. IBA and IAA @ 0 - 1.5 mg L-1 alone for root formation where 1mg L-1 IBA enriched media recorded highest number of functional roots (14.55 per explant). 70% plantlets survival was recorded during hardening.