Assessment of dermal and ocular irritation potential of soap from extracts of leaves of Morinda morindoides

Abstract

Dermal and ocular irritation potentials of soap from extracts of leaves of morindoides codified Morinda were assessed using in vivo acute irritation tests in albino rabbits. Irritation risk of this soap was performed according conventional test for assessing skin and eye irritation potentials of chemicals with Draize rabbit test. For skin irritation test, 0.5 g dose of soap was applied to intact and abraded skin approximately 2.5×2.5 cm square of six albino New-Zealand rabbits and left in place for 24 hrs. Acute eye irritation test was carried out in nine albino rabbits. Soap at concentration of 50 mg/mL amount 0.1 mL was once placed in conjunctival sac of left eye of each animal after gently pulling the lower lid way from eye ball. Soap was non-irritating on rabbit skin test with a primary irritation index of 0.125. For eye irritancy test, this soap was weakly irritating with index of acute ocular irritation index of 2.66. Results from the present study suggest that soap made from hexane extract of M. morindoides as antimicrobial agent have promising potential medicated soap that do not induce significant levels of skin and eye irritation.j